overlapping PCR

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• Variants of PCR — This page assumes familiarity with the terms and components used in the Polymerase Chain Reaction (PCR) process. The versatility of PCR has led to a large number of variants: Contents 1 Basic modifications 2 Pretreatments and extensions 3 Other… …   Wikipedia

• Polymerase chain reaction — PCR redirects here. For other uses, see PCR (disambiguation). A strip of eight PCR tubes, each containing a 100 μl reaction mixture The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a… …   Wikipedia

• Delitto perfetto — is a genetic technique for in vivo site directed mutagenesis in yeast. This name is an Italian term for perfect deletion and is also an idiom for perfect murder. The name refers to the ability of the technique to create desired genetic changes… …   Wikipedia

• Gene synthesis — is the process of synthesizing an artificially designed gene into a physical DNA sequence.Gene synthesis was first demonstrated by Har Gobind Khorana in 1970 for a short artificial gene. Nowadays, commercial gene synthesis services are available… …   Wikipedia

• Ácido desoxirribonucleico — «ADN» redirige aquí. Para otras acepciones, véase ADN (desambiguación). «DNA» redirige aquí. Para otras acepciones, véase DNA (desambiguación) …   Wikipedia Español

• Secuenciación de ADN — La secuenciación de ADN es un conjunto de métodos y técnicas bioquímicas cuya finalidad es la determinación del orden de los nucleótidos (A, C, G y T) en un oligonucleótido de ADN. La secuencia de ADN constituye la información genética heredable… …   Wikipedia Español

• Artificial gene synthesis — is the process of synthesizing a gene in vitro without the need for initial template DNA samples. The main method is currently by oligonucleotide synthesis (also used for other applications) from digital genetic sequences and subsequent annealing …   Wikipedia

• Overlap extension polymerase chain reaction — This page assumes familiarity with the terms and components used in polymerase chain reaction (PCR) process. The overlap extension polymerase chain reaction (or OE PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension… …   Wikipedia

• DNA sequencing — Part of a series on Genetics Key components Chromosome DNA • RNA Genome Heredity Mutation Nucleotide Variation …   Wikipedia

• Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

• DNA shuffling — is a way to rapidly propagate beneficial mutations in a directed evolution experiment. It is used to rapidly increase DNA library size. [1] Contents 1 Procedure 2 Shuffling methods 2.1 Restriction …   Wikipedia